Case Study and Case Report 2017; 7(3): 65 - 75.

Salmanoglu E, Kurutas EB. Effects of 99mTc-MDP bone scintigraphy on
oxidative/nitrosative stress biomarkers in patients. Case Study Case Rep. 2017; 7(3): 65 - 75

ABSTRACT
We aimed to define the effects of Technetium-99m Methylendiphosphonate bone
scintigraphy on oxidative/nitrosative stress in patients after intravenous injection and
compared to healthy control group. Forty patients for bone scintigraphy and forty healthy
control group were included this study. The age range was similar in both groups (18-74
years). The blood samples were taken from patients 2 hour after intravenous injection of 20
mCi (740 MBq) Technetium-99m Methylendiphosphonate. The activities of catalase,
superoxide dismutase and malondialdeyhde levels were measured as oxidative stress
biomarkers and nitric oxide and nitrotyrosine levels were measured as nitrosative stress
biomarkers in patients and control group. There were no significant differences according to
both age and sex between two groups (p˃0.05). Activities of catalase and superoxide
enzymes and malondialdeyhde levels in patients were increased in patients compared to
controls (p<0.05). Also, nitric oxide and nitrotyrosine levels in patients were higher than
control group (p<0.05). Technetium-99m Methylendiphosphonate may cause both oxidative
and nitrosative stress in patients. Also antioxidant defense of the patients was higher
compared to healthy subjects possibly due to a compensatory response to ionising radiation in
the living cells and thereby protects the cells against oxidative/nitrosative damage.

Keywords: Bone scintigraphy, oxidative/nitrosative stress, catalase, superoxide dismutase,
malondialdehyde, nitric oxide, nitrotyrosine

Full text available online at
https://drive.google.com/file/d/0B93jwLCAD78YYlBMV0xoLUY5SnM/view